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Clavulanic acid decreased the MICs to 8 and
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belongs to the group of strictly anaerobic gram-negative
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cocci. Fermentans strain (RYC-MR95) resistant to penicillin and expanded-spectrum cephalosporin (amoxicillin and cefotaxime
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MICs, 64 microgram/ml)
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was isolated from a human perianal abscess. The ACI-1
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enzyme (estimated pI 4.3) had. A fragment encoding a beta-lactamase from genomic DNA was cloned
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in Escherichia coli K-12 strain HB101, and the recombinant strain expressed resistance to amoxicillin (MIC, 1,024 microgram/ml) and cefotaxime (MIC, 4 microgram/ml). Analysis of the nucleotide sequence revealed a new class A beta-lactamase,
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ACI-1. All previously described Acidaminococcus strains are susceptible to beta-lactam antibiotics. In accordance with
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its biochemical properties, we propose to assign ACI-1 to functional group 2be. ACI-1 from Acidaminococcus fermentans.